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| Kremer,Frederico Schmitt; McBride,Alan John Alexander; Pinto,Luciano da Silva. |
| Abstract The introduction of next-generation sequencing (NGS) had a significant effect on the availability of genomic information, leading to an increase in the number of sequenced genomes from a large spectrum of organisms. Unfortunately, due to the limitations implied by the short-read sequencing platforms, most of these newly sequenced genomes remained as “drafts”, incomplete representations of the whole genetic content. The previous genome sequencing studies indicated that finishing a genome sequenced by NGS, even bacteria, may require additional sequencing to fill the gaps, making the entire process very expensive. As such, several in silico approaches have been developed to optimize the genome assemblies and facilitate the finishing process. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microbial genetics; Molecular microbiology; Genomics; Microbiology; Draft genomes. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400553 |
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| Vianna,Ana Muñoz; Gonçales,Relber Aguiar; Lara,Ana Paula de Souza Stori de; Pinto,Luciano da Silva; Nizoli,Leandro Quintana; Leite,Fábio Pereira Leivas. |
| A piroplasmose equina causada por Theileria equi acomete os equinos de forma endêmica no Brasil e em diversos outros países tropicais e subtropicais. Considerada uma das mais importantes doenças de equinos, causa danos à saúde animal e perdas econômicas. A proteína equi merozoite antigen (EMA-2) é uma das principais proteínas de superfície, expressa nos diversos estágios do ciclo do parasita, estimula resposta imune em animais infectados, tornando-se um possível candidato para utilização em diagnóstico. O gene EMA-2 foi clonado e expresso na levedura Pichia pastoris. A proteína EMA-2 recombinante (rEMA-2) foi caracterizada antigenicamente por Western Blot e por ELISA indireto, utilizando-se soro de equino positivo para theileriose. O resultado do ELISA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Equinos; Imunodiagnóstico; Piroplasmose; Theileriose. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014001001830 |
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| Proietti,Maíra Carneiro; Lara-Ruiz,Paula; Reisser,Júlia Wiener; Pinto,Luciano da Silva; Dellagostin,Odir Antonio; Marins,Luis Fernando. |
| We analyzed mtDNA control region sequences of green turtles (Chelonia mydas) from Arvoredo Island, a foraging ground in southern Brazil, and identified eight haplotypes. Of these, CM-A8 (64%) and CM-A5 (22%) were dominant, the remainder presenting low frequencies (< 5%). Haplotype (h) and nucleotide ( π) diversities were 0.5570 ± 0.0697 and 0.0021 ± 0.0016, respectively. Exact tests of differentiation and AMOVA ΦST pairwise values between the study area and eight other Atlantic foraging grounds revealed significant differences in most areas, except Ubatuba and Rocas/Noronha, in Brazil (p > 0.05). Mixed Stock Analysis, incorporating eleven Atlantic and one Mediterranean rookery as possible sources of individuals, indicated Ascension and Aves islands... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Foraging grounds; Genetic diversity; Green turtle; MtDNA haplotypes; Natal origins. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300027 |
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